Involvement of Krüppel-like factor 6 (KLF6) mutation in the development of nonpolypoid colorectal carcinoma

Figure 1 Representative example (case 22). A: Histologic findings. Open squares indicate microdissected areas. T1-T4: tumor samples; N: normal sample. B: Microsatellite assay of D10S591. T3 and T4 show allelic loss of the locus (arrow). C:polymerase chain reaction-single-strand conformation polymorphism analysis of KLF6. T3 and T4 show mobility shift (arrow). D: Direct sequencing analysis of KLF6. A missense mutation at codon 735 (CTG to GCT) is confirmed.