Heme oxygenase-1 overexpression increases liver injury after bile duct ligation in rats

Figure 7 Effects of cholestasis and HO-1 overexpression on collagen-Iα mRNA expression. Livers were harvested 3 wk after BDL, perfused with normal saline, and frozen in liquid nitrogen. RNase protection assays were performed using an individual probe. Values are means ± SD (n = 4 in each group). Specific bands were quantitated by scanning densitometry and normalized to the signal of GAPDH. A: representative autoradiogram of collagen-Iα mRNA expression; B: densitometric analysis of collagen-Iα mRNA expression. ^aP < 0.05 vs sham-operated groups, ^CP < 0.05 vs bile duct-ligated group receiving saline.