Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells

Figure 1 Morphological changes of BM-MSCs during differentiation. A: The third passage of pre-induced BM-MSCs (× 500); B: BM-MSCs formed islet-like clusters under 5% FBS HG-DMEM culture (× 500); C: Some clusters were half suspended in the culture medium after induced by nicotinamide and exendin-4 (× 500); Electron microscopy: Secretory granules (the black arrow shows) are densely packed within the cytoplasm of D-MSCs (E, × 4000) whereas few is found in pre-induced BM-MSCs (D × 5000); F: Surface markers of BM-MSCs showed that CD90 and CD29 positive rates were more than 93% whereas CD45 expressions were less than 1%.