Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells

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Jun 28, 2007 (publication date) through Nov 4, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 28, 2007; 13(24): 3342-3349
Published online Jun 28, 2007. doi: 10.3748/wjg.v13.i24.3342

Table 1 List of rat gene-specific primers in RT-PCR

Genes	Forward primer	Reverse primer	Annealingtemperature (°C)	GenBankaccession no.	Size of PCRproduct (bp)
InsulinI	CCGTCGTGAAGTGGAG	CAGTTGGTAGAGGGAGCAG	57	NM_019129	156
InsulinII	ATGGCCCTGTGGATCCGCTT	CTAGTTGCAGTAGTTCTCCA	53	NM_019130	333
Glucagon	ATCATTCCCAGCTTCCCAGA	CGGTTCCTCTTGGTGTTCAT	54	NM_012707	152
Somatostatin	CAGGAACTGGCCAAGTAC	AGTTCTTGCAGCCAGCTTTG	54	NM_012659	187
IAPP	AGTCCTCCCACCAACCAATGT	AGCACAGGCACGTTGTTGTA	54	NM_012586	220
GLUT-2	TTACTCTCCATTTCAGTCCTTTGT	TAGAGCAGCTCTTTATTCCAGATTT	53	J03145	165
GK	ACCAGAAAGGGGAGGCCT	ATTAAAAACTCCCCCACAGTCC	51	NM_012565	179
GLP-1R	TCCTGTTAAAGCTGCAAGGC	TTGTCCGAGAGGAAGGCTG	54	NM_012728	232
PDX-1	GGTGCCAGAGTTCAGTGCTAA	CCAGTCTCGGTTCCATTCG	53	NM_022852	249
Ngn3	CTTCACAAGAAGTCTGAGAACACCAG	CTGCGCATAGCGGACCACAGCTTC	57	NM_021700	233
NeuroD1	TGTCGTTACTGCCTTTGGAA	CGATCTGAATACAGCTACACGAA	53	NM_019218	151
PAX-6	CGACAAGATTTGCCATGGAT	CAACCTTTGGAAAAACCAACA	54	NM_013001	179
Nkx2.2	CACGCAGGTCAAGATCTG	TGCCCGCCTGGAAGGTGGCG	55	X81408	188
GAPDH	CACCCTGTTGCTGTAGCCATATTC	GACATCAAGAAGGTGGTGAAGCAG	57	NM_017008	196

IAPP: Islet amyloid polypeptide; GK: Glucokinase.

Citation: Wu XH, Liu CP, Xu KF, Mao XD, Zhu J, Jiang JJ, Cui D, Zhang M, Xu Y, Liu C. Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells. World J Gastroenterol 2007; 13(24): 3342-3349
URL: https://www.wjgnet.com/1007-9327/full/v13/i24/3342.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i24.3342