Role of matrix metalloproteinase, tissue inhibitor of metalloproteinase and tumor necrosis factor-α single nucleotide gene polymorphisms in inflammatory bowel disease

Table 3 Effect of MMP, TIMP and TNF-α allelic SNP composition on age at onset of disease, colon involvement at first endoscopic/radiologic examination and development of fistulae or stenotic strictures during follow-up [median 24.7 (range 3.3-58.5) yr] in CD

Protein	SNP	Genotype	Number ofpatients	Age (yr) at onsetof disease,median (range)	Colonic involvementat first examination(% of patients)	Fistulae duringfollow-up(% of patients)	Strictures duringfollow-up(% of patients)
MMP-1	-1607 1G/2G	1G1G	38	20.7 (0.3-45.6)	52.6	71.1	84.2
		1G2G	54	22.4 (7.0-61.5)	50	63	75.9
		2G2G	26-27	21.0 (3.4-40.2)	57.7	63	70.4
MMP-2	-1306 C/T	CC	66-67	20.1 (5.8-61.5)	57.6	64.2	74.6
		CT+TT	521	22.4 (0.3-50.1)	46.2	67.3	80.8
MMP-3	-1613 5T/6T	5T5T	34	22.4 (0.3-53.6)	35.3a	70.6	91.2a
		5T6T	54	19.3 (3.4-61.5)	63	66.7	74.1
		6T6T	30-31	23.5 (7.2-49.1)	53.3	58.1	67.7
MMP-9	-1562 C/T	CC	88-89	21.0 (0.3-61.5)	53.4	68.5	75.3
		CT+TT	301	22.7 (7.2-45.5)	50	56.7	83.3
TIMP-1, ♂	+372 T/C	T	32	18.9 (0.3-48.3)	53.1	78.1	87.5
		C	17	18.5 (5.9-61.5)	52.9	76.5	94.1
TIMP-1, ♀	+372 T/C	TT	29	24.3 (7.0-56.8)	55.2	41.4c	69.0
		TC	22-23	23.5 (3.4-39.6)	45.5	69.6	78.3
		CC	18	22.2 (10.4-49.1)	55.6	66.7	55.6
TIMP-2	+303 G/A	GG	91-92	21.5 (0.3-61.5)	56.0	68.5	77.2
		GA+AA	271	22.4 (3.4-56.8)	40.7	55.6	77.8
TNF-α	-308 G/A	GG	79-80	22.6 (0.3-61.5)	50.6	68.8	78.8
		GA+AA	391	18.9 (3.4-45.5)	56.4	59.0	74.4

¹Only 7, 1, 2 and 4 patients were carrying the MMP-2, -9, TIMP-2 or TNF-α mutant genotype, respectively, and were combined with the heterozygote group for statistical purposes. Differences in phenotype between genotypes were tested for statistical significance by Kruskal-Wallis/Mann-Whitney U (onset) or Chi-Square test (colon involvement/fistulae and stricture development).

^aP≤ 0.03 vs 5T6T and 6T6T combined,

^cP = 0.025 vs TC and CC combined.