5-aminosalicylic acid in combination with nimesulide inhibits proliferation of colon carcinoma cells in vitro

Figure 2 Time-dependent inhibitory effect of 5-ASA or nimesulide on proliferation of HT-29 colon carcinoma cells. The cells were pretreated with 100 μmol/L 5-ASA or 100 μmol/L Nimesulide respectively for 12, 24, 48, 72, 96 h), then dye uptake was measured at 490 nm using an ELISA reader. Data were expressed as mean ± SE of five independent samples. The inhibitory rate was markedly increased in a time-dependent manner.