Mechanisms involved in Korean mistletoe lectin-induced apoptosis of cancer cells

Figure 6 Inhibition of VCA-induced caspase activation and VCA-induced decrease of anti-apoptotic molecules in COLO cells treated with anti-TNFR1 antibody. COLO cells were treated without (-) or with (+) VCA (100 ng/mL) for 12 h in the presence (+) or absence (-) of anti-TNFR1 antibody (α-TNFR1, 10 μg/mL). Cell lysates were prepared and western blot was performed using specific antibodies for (A) various procaspases (PCas) and (B) anti-apoptotic molecules. Immunoreactive bands were visualized by chemiluminescence. Representative western blot is shown (left panels). Each band was quantified and normalized against the density of the internal control (actin). Normalized values were used to calculate the relative expression as a percentage of COLO cells without VCA or anti-TNFR1 antibody treatment. Values are mean ± SE of three independent experiments (right panels). ^aP < 0.05 vs VCA-treated cells without anti-TNFR1.