Mechanisms involved in Korean mistletoe lectin-induced apoptosis of cancer cells

Figure 4 Decreased expression of anti-apoptotic molecules in COLO cells treated with VCA. COLO cells were treated without (-) or with (+) VCA (100 ng/mL) for the indicated times. Cell lysates were prepared and western blots were performed using specific antibodies. Immunoreactive bands were visualized by chemiluminescence. Representative western blots are shown (A). Each band was quantified and normalized against the internal control (^®-actin). Normalized values were used to calculate the relative expression as a percentage of COLO cells without VCA treatment at each time point (B). p-Akt: phosphorylated Akt. Results are representative of three independent experiments.