Mechanisms involved in Korean mistletoe lectin-induced apoptosis of cancer cells

Figure 1 Cytotoxic effects of VCA on COLO cells in vitro. A: Cells were treated with VCA for 24 h and cell viability was determined by MTT assay. Values are mean ± SE; n = 4/group; B: COLO cells were incubated with the indicated amounts of VCA for various times and cell viability was determined by MTT assay. Values are mean ± SE, n = 5/group. ^aP < 0.05 vs control group; C: COLO cells were treated with or without VCA (100 ng/mL) for 9 h. Cells were harvested, fixed in ethanol, and stained with propidium iodide in the presence of RNase A. The stained cells were analyzed by flow cytometry.