Rescue of the albino phenotype by introducing a functional tyrosinase minigene into Kunming albino mice

Figure 1 The structure of tyrosinase minigene construct TyBS used for microinjection. The construct contains a 2.25-kb tyrosinase promoter, i.e., 5’ non-coding flanking sequence of mouse tyrosinase, as a single thick line plus 65 bp of tyrosinase exon I (to the Xho I site) and 1.785-kb Xho I-EcoR I fragment (shaded) (derived from Tyrs-J) containing tyrosinase cDNA and 3’ non-coding flanking sequence. TyBS encodes cysteine at amino acid 103 and glycine at amino acid 346. The 4.1-kb injected fragment was obtained by pTyBS digestion with Kpn I and EcoR I. The restriction sites are: E, EcoR I; K, Kpn I. The primers specific for TyBS used in PCR reaction (small arrows) and the expected size of PCR products are indicated.