TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation

Figure 3 A: Multiple fields were photographed at 0 min and 30 min. Pictures were taken at 10 × magnification and are representative of four experiments performed in triplicate. DLD-1 (U6 siRNA-TTYH2 plasmids) (lower panel) show increased cell-cell aggregation compared with control cells (upper panel); B: The rate of aggregation potential was calculated as the percentage of the number of single cells in a microscope field using the formula [(No - N1)/No] × 100, where No is the total number of cells and N1 is the number of single cells detected in the cultures at the different incubation times. The data represent the means ± SD of four experiments.