Promoter hypermethylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil

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May 14, 2007 (publication date) through Nov 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. May 14, 2007; 13(18): 2568-2574
Published online May 14, 2007. doi: 10.3748/wjg.v13.i18.2568

Table 1 Primer sequences (5'-3') for MSP

Gene	Sense	Antisense	Product size	TM
CDH1	M-ATTCGAATTTAGTGGAATTAGAATC	M-CCCAAAACGAAACTAACGAC	125 bp	53°C
CDH1	U-GGATTTGAATTTAGTGGAATTAGAATT	U-CTCCCCAAAACAAAACTAACAAC	130 bp	53°C
FHIT	M-TTTTCGTTTTTGTTTTTAGATAAGC	M-AAAAATATACCCACTAAATAACCGC	157 bp	52°C
FHIT	U-TGGTTTTTGTTTTTGTTTTTAGATAAGT	U-AAAATATACCCACTAAATAACCACC	159 bp	52°C
MTAP	M-TGTTTTTTAGGAATTAAGGGAAATAC	M-AACTACAAAATCTAACCCGACGAC	199 bp	52°C
MTAP	U-TTTTTAGGAATTAAGGGAAATATGT	U-CAACTACAAAATCTAACCCAACAAC	197 bp	52°C
PLAGL1	M-GTTTATTTTGGCGGAGATTTC	M-ACTAAACGACACCCACACGTC	147 bp	51°C
PLAGL1	U-GGTTTATTTTGGTGGAGATTTTG	U-AAAAACTAAACAACACCCACACAT	152 bp	51°C

M: methylated sequences; U: unmethylated sequences; TM: melting temperature.

Citation: Leal MF, Lima EM, Silva PNO, Assumpção PP, Calcagno DQ, Payão SLM, Burbano RR, Smith MAC. Promoter hypermethylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil. World J Gastroenterol 2007; 13(18): 2568-2574
URL: https://www.wjgnet.com/1007-9327/full/v13/i18/2568.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i18.2568