High expression of hepatitis B virus based vector with reporter gene in hepatitis B virus infection system

Figure 3 PHH were detected by FACS. The purity of PHH cultures was analyzed by identification of nonparenchymal and parenchymal liver cells on d 6 after seeding. A: Control; B: FACS analysis of parenchymal liver cells with GFP; C: FACS analysis of kupffer cells with Dextran conjugates; D: FACS analysis of liver sinusoidal endothelial cells (LSEC) with acetylated low-density lipoprotein. The population consisted of 10.4% AcLDL positive cells, 2% Carboxy-Q-Rhodamine positive cells and 75.2% GFP positive cells, meaning that total parenchymal cells is about 87.6%, approximately 85% of it were infected by HBV based vector with GFP.