Protein and non-protein sulfhydryls and disulfides in gastric mucosa and liver after gastrotoxic chemicals and sucralfate: Possible new targets of pharmacologic agents

Table 5 Protein disulfides (PSSP) in rat glandular stomach mucosa and liver following various chemicals given i.g.

	0 min	1 min	3 min	6 min	12 min
Stomach
Ethanol	0.55 ± 0.08	0.94 ± 0.06b	1.36 ± 0.20b	1.72 ± 0.05d	1.71 ± 0.13d
HCl	0.54 ± 0.20	0.57 ± 0.04a	0.90 ± 0.30	1.33 ± 0.45	1.69 ± 0.09b
NaOH	0.44 ± 0.07	0.52 ± 0.17	0.55 ± 0.04	0.66 ± 0.14	0.59 ± 0.20
NaCl	0.57 ± 0.02	0.53 ± 0.02	1.00 ± 0.12b	1.01 ± 0.18b	1.35 ± 0.05d
Ammonia	0.39 ± 0.07	0.52 ± 0.17	0.56 ± 0.15	0.78 ± 0.20	0.64 ± 0.15
Liver
Ethanol	0.89 ± 0.25	0.75 ± 0.20	0.95 ± 0.30	0.86 ± 0.17	0.69 ± 0.05

The damaging agent, 1 mL of 75% ethanol, 0.6 mol/L HCl, 0.2 mol/L NaOH, 25% NaCl or 1% ammonia-water was given by gavage to fasted S-D rats (n = 4-6), and the animals were killed subsequently at time-points indicated. Data are expressed as mean ± SEM of nmol half cystine residue in 1 mg protein.

^bP < 0.01,

^dP < 0.001, vs corresponding control values (0 min).