Protein and non-protein sulfhydryls and disulfides in gastric mucosa and liver after gastrotoxic chemicals and sucralfate: Possible new targets of pharmacologic agents

Table 4 Protein-GSH mixed disulfides (PSSG) in rat glandular stomach mucosa and liver following various chemicals given i.g.

	0 min	1 min	3 min	6 min	12 min
Stomach
Ethanol	0.17 ± 0.04	0.28 ± 0.08	0.37 ± 0.03b	0.35 ± 0.02b	0.49 ± 0.04d
HCl	0.20 ± 0.02	0.62 ± 0.11a	0.83 ± 0.15a	0.83 ± 0.16a	0.84 ± 0.09b
NaOH	0.22 ± 0.03	0.31 ± 0.10	0.36 ± 0.09	0.45 ± 0.12	0.43 ± 0.15
NaCl	0.20 ± 0.02	0.19 ± 0.04	0.17 ± 0.04	0.20 ± 0.01	0.22 ± 0.05
Ammonia	0.26 ± 0.03	0.25 ± 0.04	0.27 ± 0.04	0.36 ± 0.11	0.32 ± 0.04
Liver
Ethanol	0.68 ± 0.03	0.86 ± 0.03	0.74 ± 0.03	0.64 ± 0.11	0.81 ± 0.10

The damaging agent, 1 mL of 75% ethanol, 0.6 mol/L HCl, 0.2 mol/L NaOH, 25% NaCl or 1% ammonia-water was given by gavage to fasted S-D rats (n = 4-6), and the animals were killed subsequently at time-points indicated. Data are expressed as mean ± SEM of nmol GSSG in 1 mg protein.

^aP < 0.05,

^bP <0.01,

^dP < 0.001, vs corresponding control values (0 min).