Protein and non-protein sulfhydryls and disulfides in gastric mucosa and liver after gastrotoxic chemicals and sucralfate: Possible new targets of pharmacologic agents

Table 3 Protein SH (PSH) in the mucosa of rat glandular stomach and liver following i.g. administration of gastrotoxic chemicals

	0 min	1 min	3 min	6 min	12 min
Stomach
Ethanol	32.9 ± 1.2	29.6 ± 1.9	22.4 ± 1.1b	15.9 ± 1.3d	13.7 ± 1.0b
HCl	34.2 ± 3.0	39.8 ± 2.1	38.7 ± 1.3	35.7 ± 3.3	31.7 ± 4.2
NaOH	26.9 ± 1.0	22.3 ± 2.1	13.0 ± 4.1b	11.8 ± 3.5b	14.0 ± 4.9a
NaCl	26.7 ± 3.6	26.5 ± 2.1	23.1 ± 6.9	13.0 ± 6.7	15.1 ± 2.1a
Ammonia	29.8 ± 2.9	22.6 ± 2.0	26.6 ± 3.9	30.9 ± 2.1	26.3 ± 4.9
Liver
Ethanol	55.4 ± 4.8	51.2 ± 2.4	53.0 ± 1.8	54.3 ± 4.1	50.6 ± 4.5

The damaging agent, 1 mL of 75% ethanol, 0.6 mol/L HCl, 0.2 mol/L NaOH, 25% NaCl or 1% ammonia-water was given by gavage to fasted S-D rats (n = 4-6), and the animals were killed subsequently at time-points indicated. Data are expressed as mean ± SEM of nmol cysteine in 1 mg protein.

^aP < 0.05,

^bP < 0.01,

^dP < 0.001, vs corresponding control values (0 min).