Protein and non-protein sulfhydryls and disulfides in gastric mucosa and liver after gastrotoxic chemicals and sucralfate: Possible new targets of pharmacologic agents

Table 1 Total glutathione (GSH + GSSG) in the mucosa of rat glandular stomach and liver following i.g. administration of gastrotoxic chemicals

	0 min	1 min	3 min	6 min	12 min
Stomach
Ethanol	2.6 ± 0.3	1.7 ± 0.5	1.6 ± 0.3	1.4 ± 0.3a	1.2 ± 0.2b
HCl	2.3 ± 0.7	1.5 ± 0.6	0.9 ± 0.4	0.9 ± 0.3	0.5 ± 0.2a
NaOH	2.6 ± 0.5	2.4 ± 0.4	1.9 ± 0.6	1.9 ± 0.6	1.9 ± 0.5
NaCl	2.9 ± 0.6	2.5 ± 0.5	2.0 ± 0.2	1.8 ± 0.1	1.5 ± 0.3a
Ammonia	3.0 ± 0.7	2.6 ± 0.5	2.3 ± 0.4	2.8 ± 0.4	2.9 ± 0.7
Liver
Ethanol	3.8 ± 0.5	3.9 ± 1.0	4.1 ± 0.6	4.3 ± 0.8	4.2 ± 0.5

The damaging agent, 1 mL of 75% ethanol, 0.6 mol/L HCl, 0.2 mol/L NaOH, 25% NaCl or 1% ammonia-water was given by gavage to fasted S-D rats (n = 4-6), and the animals were killed subsequently at time-points indicated. Data are expressed as mean ± SEM of nmol GSH mg wet tissue.

^aP < 0.05,

^bP < 0.01, vs corresponding control values (0 min).