Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China

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Apr 7, 2007 (publication date) through Sep 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 7, 2007; 13(13): 1975-1979
Published online Apr 7, 2007. doi: 10.3748/wjg.v13.i13.1975

Table 2 Probe sequence used for HBV genotyping

Genotype	Oligonucleotide sequence	Tm (°C)
A	5’-NH₂-(T)₁₆-AAC CCC (A/G)TC AAG GAC CAC TG-3’	56
B	5’-NH₂-(T)₁₆-CCC TGC ATT CAA AGC CAA CTC-3’	56
C	5’-NH₂-(T)₁₆-CTT CAA CCC CAA CAA GGA TCA-3’	54
D	5’-NH₂-(T)₁₆-CAA TCC CAA CAA GGA CAC CTG-3’	55
E	5’-NH₂-(T)₁₆-GAC CAC AAT CCC AAC AAA GAC C-3’	55
F	5’-NH₂-(T)₁₆-TGG CCA ATG GCA AAC AAG G-3’	54
G	5’-NH₂-(T)₁₆-CAA TCC CAA AAA GGA CCC TTG-3’	54
H	5’-NH₂-(T)₁₆-CTC TCA ACG GCG AGA AGG G-3’	54
QC probe	5’-NH₂-(T)₁₆-TGG GGT GGA GCC CTC AG-3’	59
PC probe	5’-NH₂-(T)₁₆- ATC CTC AGG CCA TGC AGT G-3’	58

Citation: Tang XR, Zhang JS, Zhao H, Gong YH, Wang YZ, Zhao JL. Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China. World J Gastroenterol 2007; 13(13): 1975-1979
URL: https://www.wjgnet.com/1007-9327/full/v13/i13/1975.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i13.1975