Cloning and expression of special F protein from human liver

Figure 8 SDS-PAGE of target protein in purity process. Lane 1: protein induced from bacteria; lane 2: inclusion body precipitation of all bacteria after cleavage and centrifugation; lane 3: dissolved inclusion granule after preliminary purification; lane 4: rough protein eluted by affinity chromatography; lane 5: chromatography effluent before the target protein was eluted; lane 6: first pipe of aim effluent; lane 7: second pipe of aim effluent; lane 8: last pipe of aim effluent; lane 9: blank control (the blank strain containing no expression vector).