Cloning and expression of special F protein from human liver

Figure 6 Digestion and identification of expression vector. Pet15b-F. Lane 1: DNA Marker (DL15000); lane 2: single enzyme digestion of helper plasmid pLysS in expressed strain (pLysS/HindIII); lane 3: single enzyme digestion of expression vector pET15b-F containing the exogenous gene (pET15b-F/HindIII); lane 4: double enzyme digestion of the blank vector pET-15b-pET-15b/BamHI + NdeI); lane 5: double enzyme digestion of the expression vector pET15b-F containing the exogenous gene (pET15b-F/BamHI + NdeI; lane 6: double enzyme digestion of cloning vector pUCm-T-F containing the exogenous gene (pUCm-T/BamHI + NdeI; lane 7: double enzyme digestion of helper plasmid pLysS in the expressed strain (pLysS/BamHI + NdeI).