Upregulation of hypoxia inducible factor 1α mRNA is associated with elevated vascular endothelial growth factor expression and excessive angiogenesis and predicts a poor prognosis in gastric carcinoma

Figure 2 The expression of VEGF protein in gastric cancer tissue. A: VEGF was negative (-) in nontumorous gastric epithelial mucous. In situ hybridization and visualization with DBA. Magnification × 120; B: Poorly differentiated adenocarcinoma was involved with muscular layer. VEGF was positively expressed (+++). In situ hybridization and visualization with DAB. Magnification ×180; C: Moderately differentiated adenocarcinoma was involved with serosa layer. HIF-1αmRNA was positively expressed (+++). In situ hybridization and visualization with DAB. Magnification × 200; D: Poorly differentiated adenocarcinoma was involved with great omentum. VEGF was positively expressed (++). In situ hybridization and visualization with DAB. Magnification × 220.