Upregulation of hypoxia inducible factor 1α mRNA is associated with elevated vascular endothelial growth factor expression and excessive angiogenesis and predicts a poor prognosis in gastric carcinoma

Figure 1 The expression of HIF-1α mRNA in gastric cancer tissue. A: HIF-1α mRNA was negative (-) in nontumorous gastric epithelial mucous. In situ hybridization and visualization with DBA. Magnification × 120; B: Moderately differentiated adenocarcinoma was involved with muscular layer. HIF-1α mRNA was positively expressed (+++). In situ hybridization and visualization with DAB. Magnification × 180; C: Moderately differentiated adenocarcinoma was involved with serosa layer. HIF-1α mRNA was positively expressed (++). In situ hybridization and visualization with DAB. Magnification × 210; D: Moderately differentiated adenocarcinoma was involved with great omentum. HIF-1α mRNA was positively expressed (++). In situ hybridization and visualization with DAB. Magnification × 240.