Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method

Figure 4 Efficiency of IPC formation. A: Ratio of DsRed- expressed cells 4 (before nucleofection), 8, 12 and 18 d after experiment in the groups of cells with pax-4, mock and pax-4 siRNA plasmid; B: Insulin secretion in medium detected by ELISA; C: Insulin content in cells after 24 h incubation with different concentrations of glucose (5 mmol/L, 15 mmol/L or 30 mmol/L) and with/without 10 μmol/L Tolbutamide treatment (T).