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©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 21, 2007; 13(11): 1672-1679
Published online Mar 21, 2007. doi: 10.3748/wjg.v13.i11.1672
Published online Mar 21, 2007. doi: 10.3748/wjg.v13.i11.1672
Figure 2 Nucleofection of EB-dissociated cells.
A: EB-dissociated cells after four days by hanging drop; B: eGFP fluorescent view (Bar= 50 μm in A, B); C: Morphology of EB-dissociated cells 56 h after nucleofection; D: eGFP filter view; E: DsRed view and the merge image (Bar = 5 μm in C-E); F: Efficiency of gene delivery; G: Latency of gene examination in ES cells after nucleofection. The ratio of DsRed-expressed cells was counted by random photograph 8, 12 and 18 d after experiment.
- Citation: Lin HT, Kao CL, Lee KH, Chang YL, Chiou SH, Tsai FT, Tsai TH, Sheu DC, Ho LL, Ku HH. Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method. World J Gastroenterol 2007; 13(11): 1672-1679
- URL: https://www.wjgnet.com/1007-9327/full/v13/i11/1672.htm
- DOI: https://dx.doi.org/10.3748/wjg.v13.i11.1672