Sonic hedgehog-Gli1 pathway in colorectal adenocarcinomas

Figure 4 Expression of Gli1, Gli3 and Hip in primary colorectal adenocarcin-omas. We detected expr-ession patterns of Gli1, Gli3 and Hip transcripts by in situ hybridization (positive in blue). Gli1 (A) and Hip (E) were not expressed in normal control tissue. B: Gli1 was expressed in cytop-lasm of malignant crypt. The expression of Gli1 (C) and Hip (D) sense probe in tumor tissue. F: Hip was expressed in cytoplasm of tumor cells. G: Gli3 was not expressed in colorectal adenocarcinomas. H is the normal control of Gli3 and I is the sense control of Gli3.