Sonic hedgehog-Gli1 pathway in colorectal adenocarcinomas

Figure 3 Expression of Ptch1 in primary colorectal adenocarcinomas. Ptch1 transcript (blue as positive) was detected by in situ hybridization in the normal control (A) and colorectal cancers (B). C is from the same tumor with B being derived from the Ptch1 sense probe. To confirm the in situ hybridization results, we performed immunohistochemistry with PTCH1 antibodies, showing positive staining of PTCH1 protein (positive in brown) in malignant crypts (E). D is the normal control and F is the negative control.