Sonic hedgehog-Gli1 pathway in colorectal adenocarcinomas

Figure 2 Expression of Shh in primary colorectal adenocarcinomas. In situ hybridization was performed to detect Shh transcript in normal (A) and cancerous (B) tissues (positive in blue), and the sense probe did not reveal any positive signals (C is the sense control of B). Results of in situ hybridization were confirmed by immunohistostaining in normal (D) and tumor (E) tissues (positive in brown, F is the negative control) and by RT-PCR (G).