Roles of the MEK1/2 and AKT pathways in CXCL12/CXCR4 induced cholangiocarcinoma cell invasion

Figure 6 A: Effect of AMD3100 on CXCL12-induced phosphorylation of MEK1/2 and Akt in cholangiocarcinoma cells. RMCCA1 cells were pre-treated with or without AMD3100 before added CXCL12. Cells were collected at 5 min and MEK1/2 and Akt phosphorylation were determined by Western blot as described; B: The average band intensity based on 3 biologically separate experiments, showing levels of MEK1/2 and Akt phosphorylation relative to the levels of MEK1/2 and Akt expression from the same experiment (^aP < 0.05 vs 0 ng/mL of CXCL12).