Roles of the MEK1/2 and AKT pathways in CXCL12/CXCR4 induced cholangiocarcinoma cell invasion

Figure 4 Effect of CXCL12 on the invasion of cholangiocarcinoma cells. RMCCA1 and KKU100 were pre-treated with or without AMD3100 for 12 h then were seeded in the 24-well Biocoat Matrigel invasion chamber. The bottom chamber contained 0 or 100 ng/mL of CXCL12. After 24 h, the cells on the lower surface were assayed as described previously. CXCL12 induced the invasion of cholangiocarcinoma cells. The effect of CXCL12 was decreased when the cells were pre-treated with AMD3100 (^aP < 0.05).