Epithelial cells with hepatobiliary phenotype: Is it another stem cell candidate for healthy adult human liver?

Figure 1 Morphology of LECs after liver cells primary culture (× 200). A: After 15 d primary culture, small colonies of LECs emerged; B: organized in monolayers; C: When colonies were evident, the other cells types were mechanically eliminated and LECs were lifted and replated; D: LEC population reaching 70% confluence analyzed for the other passages.