Th immune response induced by H pylori vaccine with chitosan as adjuvant and its relation to immune protection

Table 5 Levels of IL-2, IFN-γ, IL-12, IL-10 and IL-4 in gastric mucosa after challenge (mean ± SD)

Groups	n	IL-2	IFN-γ	IL-12	IL-10	IL-4
(1) Control	10	34.3 ± 11.8	48.7 ± 27.8	233.7 ± 125.7	86.4 ± 38.2	3.87 ± 1.99
(2) Chi-solution	11	31.8 ± 27.4	56.8 ± 26.1	308.5 ± 178.7	100.7 ± 52.6	4.47 ± 1.89
(3) Chi-particles	10	51.1 ± 42.6	58.8 ± 28.1	311.3 ± 129.6	88.4 ± 51.7	7.21 ± 4.02g
(4) Hp antigen	9	97.9 ± 64.2a	59.4 ± 15.0	319.4 ± 136.3	89.3 ± 29.01	4.89 ± 3.15
(5) Hp antigen + CT	10	80.9 ± 60.2c	93.6 ± 23.5ef	487.0 ± 289.3ef	81.8 ± 49.5	3.67 ± 1.76
(6) Hp antigen + chi-solution	10	124.3 ± 75.2b	107.5 ± 42.0d	525.6 ± 112.2d	108.5 ± 39.0	8.78 ± 4.96hi
(7) Hp antigen + chi-particles	10	88.6 ± 57.0a	105.9 ± 48.1d	554.0 ± 164.4d	93.1 ± 39.2	6.59 ± 1.38k
		F = 3.370	F = 6.346	F = 5.448	F = 0.745	F = 3.214
		P = 0.002	P < 0.001	P < 0.001	P > 0.05	P = 0.003

^aP < 0.05 vs (1) and (2) groups;

^bP < 0.001 vs (1)-(3) groups;

^cP < 0.05 vs (1) group;

^dP < 0.01 vs (1)-(4) groups;

^eP < 0.05 vs (2)-(4) groups;

^fP < 0.01 vs (1) group;

^gP < 0.05 vs (1), (2) and (5) groups,

^hP < 0.01 vs (1), (2) and (5) groups;

ⁱP < 0.05 vs (4) group;

^kP < 0.05 vs (5) group; chi = chitoson.