Hepatitis B virus replication

Figure 5 Domain structure of P protein. A: Authentic DHBV P protein. Numbers are aa positions for DHBV P protein. The priming Tyr residue Y96 is indicated; B: Typical recombinant P protein construct. For solubility, a heterologous solubility enhancing domain (SED) such as NusA, GrpE, or GST is required, and a short stretch of C terminal aa must be removed. Deletion of the spacer has no negative effects on in vitro activity; C: Mini-RT2. This heavily truncated recombinant DHBV P protein requires mild detergent, but no chaperones for priming activity.