Detection of YMDD mutants using universal template real-time PCR

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Feb 28, 2006 (publication date) through Nov 4, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 28, 2006; 12(8): 1308-1311
Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1308

Table 1 Primers and probe used in universal RT-PCR

Primers or probe	Sequence
Reverse primers
Reaction V	5’-CCCCCAATACCACATCATCC-3’
Reaction I	5’-CCCCCAATACCACATCATCA-3’
Reaction C	5’-CCCCCAATACCACATCATC-3’
Forward primers	5’-tgaggagcacgagacggaagtATACAA CACCTGTATTCCCATCCCAT-3’
Probe	5’-FAM ACTTCCGTCTCGTGCTCCTCA TAMRA-3’

Citation: Wang RS, Zhang H, Zhu YF, Han B, Yang ZJ. Detection of YMDD mutants using universal template real-time PCR. World J Gastroenterol 2006; 12(8): 1308-1311
URL: https://www.wjgnet.com/1007-9327/full/v12/i8/1308.htm
DOI: https://dx.doi.org/10.3748/wjg.v12.i8.1308