Flow cytometry assay of myeloid dendritic cells (mDCs) in peripheral blood during acute hepatitis C: Possible pathogenetic mechanisms

Figure 1 Gate strategies for the mDCs CD11c+ evaluation. The letter’s order represents the sequential gates used to measure the frequency of mDCs. (A) Side scatter vs forward scatter gate analysis; (B) CD14+ cells analysis vs side scatter to select CD14+ cells avoiding debris; (C) characterization of the cells having high expression of ILT3, as marker of purified myeloid dendritic lineage, on CD14+ subset; (D) evaluation of cells expressing CD11c+, as marker of mature myeloid dendritic cells, on the whole ILT3 gated cells to obtain the mDCs; and (E) histogram of CD14-FITC mAb fluorescence.