Chronic low vitamin intake potentiates cisplatin-induced intestinal epithelial cell apoptosis in WNIN rats

Figure 1 Apoptotic index and caspase-3 activity of the intestinal mucosa of control and vitamin restricted rats treated with cisplatin or saline (vehicle). Vertical bars are means with standard error, of IEC apoptotic indices in villus (Panel A) and crypt (Panel B) regions of control, vitamin restricted rats treated with saline and cisplatin (n = 6 per group). Intestinal sections were stained with hematoxylin and eosin and studied by morphometry under a light microscope. Caspase-3 activity (Panel C) was measured in 12000 g supernatant using a colorimetric substrate.