PI 3-kinase pathway is responsible for antiapoptotic effects of atrial natriuretic peptide in rat liver transplantation

Figure 5 Effect of ANP pretreatment on phosphorylation of Bad at Ser112 and PKA-activity. A: Animals received an intravenous infusion of NaCl (Co) or ANP (5 μg/kg) for 20 min. Homogenized livers were immunoprecipitated using anti-Bad antibody. Examination of phospho-Bad (Ser112) was done via Western blotting. Data obtained in densitometric analysis were presented as mean ± SE, n = 3 in each group. Co was set as 100%. B: PKA activity was determined in liver homogenates used under (A) by in vitro phosphorylation as described. Results were expressed as percent of PKA activity in untreated livers and mean ± SE, n = 4 in each group.