Expression, purification and characterization of enterovirus-71 virus-like particles

Figure 5 Purification and characterization of EV71 VLP (100 000 X magnification,). The VLPs were produced by infecting 150 mL Sf-9 cells (1.5×10⁶ cells/mL) with Bac-P1-3CD at MOI 10, harvested at 2 dpi and purified by ultracentrifugation. The purified VLPs were characterized by SDS-PAGE (A), Western blot (B), TEM examination (C) and immunogold labeling (D). The PAGE gel was stained by Commassie blue and scanned for densitometry analysis. The primary antibody used in the Western blot and immunogold labeling was anti-VP1 MAb. The size of the gold particles was 5 nm. Bar: 100 nm.