Expression, purification and characterization of enterovirus-71 virus-like particles

Figure 1 Construction of three recombinant baculovirus vectors using pFastBac^TM DUAL plasmid as the backbone. The gene fragments encoding P1 and 3CD were cloned separately into MCS I and II of pFastBac^TM DUAL under the control of polyhedrin (P_polh) and p10 promoters (P_p10), respectively. The resultant plasmids were designated pDual-P1 and pDual-3CD, respectively. The P1 and 3CD genes were cloned together into MCS I and II under the control of polyhedrin and p10 promoters. The resultant plasmid was designated pDual-P1-3CD.