Inhibitory effects of interferons on pancreatic stellate cell activation

Figure 2 Effects of IFN and simvastatin on PSC survival. PSCs growing in 12-well plates were cultured with IFN-α (10⁶ U/L), IFN-β (10⁶ U/L), IFN-γ (100 μg/L) and simvastatin (Sim; 5 μmol/L) as indicated. To determine the percentage of cell death by flow cytometry analysis, cells with DNA content lower than the G₁ peak (sub-G₁) were considered apoptotic. Data are indicated as mean ± SE (n = 6 separate cell preparations from one rat). ^aP < 0.05 vs control cultures. Three experiments using cells from different rats yielded qualitatively identical results.