Characterization of H+ and HCO3- transporters in CFPAC-1 human pancreatic duct cells

Figure 7 Localization of Cl^–/HCO₃^– exchangers in CFPAC-1 cells. The figure shows representative pH_i traces (n = 6). The localization of Cl^–/HCO₃^– exchangers was performed by measuring the effect of changes in Cl^– gradient across the luminal or basolateral membranes on pH_i. In the presence of HCO₃^–, the removal of Cl^– from the apical membrane did not result in changes of pH_i. The removal of Cl^– from the basolateral membrane caused a ∆pH_i of 0.08 ± 0.01 and a J(B) of 4.37 ± 0.46 mmol/L B/min. Furthermore, the basolateral administration of 250 µmol/L H₂-DIDS completely blocked pH_i changes caused by the withdrawal of Cl^–.