Characterization of H+ and HCO3- transporters in CFPAC-1 human pancreatic duct cells

Figure 5 Basolateral HCO₃^– uptake – effects of H₂-DIDS, acetazolamide and varying extracellular K⁺ concentration. The figure shows representative pH_i traces. A: The anion transport inhibitor 600 µmol/L H₂-DIDS (added to the basolateral membrane of cells for 2 min before and during the administration of basolateral HCO₃^–/CO₂, dashed line, n=6) significantly decreased the extent of alkalinization (∆pH_i) and rate of J(B) (n=6); B: Varying extracellular [K⁺] caused significant differences in ∆pH_i and J(B) (n=9–11); C: The application of the membrane-permeable carbonic anhydrase inhibitor acetazolamide (100 µmol/L, n = 6) significantly decreased the overall ∆pH_i and J(B). HS: HEPES.