Variable expression of cystatin C in cultured trans-differentiating rat hepatic stellate cells

doi:10.3748/wjg.v12.i5.731

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Feb 7, 2006 (publication date) through Dec 3, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 7, 2006; 12(5): 731-738
Published online Feb 7, 2006. doi: 10.3748/wjg.v12.i5.731

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Figure 6 TGF-β1-induced CysC secretion in HSCs. Serum-starved HSCs were treated with TGF-β1 (10 ng/mL) for 24 or 48 h and the cell-free supernatants were subjected to immunoprecipitation using goat antibody P-14 directed against CysC. Subsequently, the precipitates were analyzed for CysC expression by Western blotting using an independent primary rabbit antibody directed against CysC and visualized with a goat anti-rabbit IgG-HRP conjugate.

Citation: Gressner AM, Lahme B, Meurer SK, Gressner O, Weiskirchen R. Variable expression of cystatin C in cultured trans-differentiating rat hepatic stellate cells. World J Gastroenterol 2006; 12(5): 731-738
URL: https://www.wjgnet.com/1007-9327/full/v12/i5/731.htm
DOI: https://dx.doi.org/10.3748/wjg.v12.i5.731