GFAP promoter directs lacZ expression specifically in a rat hepatic stellate cell line

Figure 8 Time-dependent induction of the endogenous GFAP gene by TGF-b1. T6/GFAP-LacZ cells were incubated with 1 ng/mL of TGF-b1 in low serum DMEM for various times (0, 8, 16, 24, 48, and 72 h), and the endogenous rat GFAP mRNA was quantified with real-time RT-PCR. Data were presented as mean ± SE. ^bP < 0.01, ^aP < 0.005 and ^dP < 0.001, when compared to treatment at 0 h.