GFAP promoter directs lacZ expression specifically in a rat hepatic stellate cell line

Figure 3 Verification of GFAP-lacZ transgene integration into host genome and legitimate expression in appropriate cell types. A: PCR of genomic DNA isolated from the transfected T6 (lane 3), C6 (lane 5) and HeLa (lane 7) showed the presence of a transgene band at 944 bp, and the absence of such band from the nontransfected T6 (lane 2), C6 (lane 4), and HeLa (lane 6); B: RT-PCR of total RNAs demonstrated that transgene only expressed in the transfected T6 (lane 3) and C6 (lane 5), but not in the transfected HeLa (lane 7); C: RT-PCR of β-actin as a reference ensuring equal sample loading. Lane 1 is the 1-kb ladder marker.