Effect of increased hepatic platelet activating factor and its receptor portal hypertension in CCl4-induced liver cirrhosis

Figure 3 Effect of cirrhosis on hepatic PAF binding. A: Results of the saturation binding assay. ³H-PAF was incubated with 100 μg membrane protein, in the presence or absence of 10 μmol/L at 30 °C for 1 h; B: Scatchard plot analysis of ³H-PAF binding to hepatic tissue of cirrhotic and control rats. Values are mean of separate experiments. Cirrhosis: R = 0.98, Kd = 8.013 nmol/L, Bax = 2.8 ± 0.213 fmol/μg membrane protein; control: R = 0.99, Kd = 5.794 nmol/L, Bax = 0.9 ± 0.061 fmol/μg membrane protein.