STI571 (Glivec) suppresses the expression of vascular endothelial growth factor in the gastrointestinal stromal tumor cell line, GIST-T1

Figure 1 4×10⁶ of GIST-T1 cells were treated with STI571 (1 μg/mL) for 1 h. Treated cells were lysed in RIPA buffer and immunoprecipitation with anti-c-KIT antibody as described in “Materials and methods”. Western blotting was performed using anti-phosphotyrosine antibody (upper panel) or anti-c-KIT antibody (lower panel). Lane 1, non-treated; lane 2, STI571 1 μg/mL. WB, Western blot; IP, immunoprecipitation.