Construction of a fusion protein expression vector MK-EGFP and its subcellular localization in different carcinoma cell lines

Figure 6 Subcellular localization analysis of MKS-GFP and MKN-GFP fusion proteins in carcinoma cell lines. Twenty-four hours after transfection HepG2 cells (upper pannel)and DU145 cells (bottom pannel) were separately transfected with EGFP-MKS and EGFP-MKN. MKS-GFP and MKN-GFP fusion proteins were localized to nucleus and accumulated in nucleolus, but rarely localized to cytoplasm. The high light “spots” in the center of nuclei are nucleoli. HepG2 cells (upper panel) and DU145 cells (bottom panel) were also transfected with pEGFP-N2 for control. It seemed that GFP protein could diffuse freely in the whole cell except the nucleoli. The whole cell is light and the low light “spots” are nucleoli.