New multi protein patterns differentiate liver fibrosis stages and hepatocellular carcinoma in chronic hepatitis C serum samples

Figure 4 Isolation of the 6. 6 kDa peak. First line shows spectrum after fractionation of the sample with Q Ceramic HyperD^®F spin columns, the second line shows the spectra after purification using reverse phase chromatography. The last line spectra represent the purified biomarker on a NP20 array after SDS-gel purification and passive elution. The arrow indicates the 6.6 kDa peak (A); One-dimensional SDS polyacrylamide gel electrophoresis after staining of enriched sample (second lane). First lane: molecular marker proteins (See Blue^® Plus2, Invitrogen, Carlsbad, USA). The arrow indicates the 6.6 kDa protein (B).