Effects of gastrin 17 on β-catenin/Tcf-4 pathway in Colo320WT colon cancer cells

Figure 2 Immunoprecipitation of β-catenin in TX-100 solution fraction and the insoluble fraction of Colo320WT cells treated with G17 (10^-8 mol/L). TX-100 solution fraction was immunoprecipitated with anti-E-cadherin antibody, while TX-100 insoluble fraction was immunoprecipitated with anti-β-catenin antibody. E-cadherin immunoprecipitate and β-catenin immunoprecipite were separated on 100 g/L SDS/PAGE gel and probed with β-catenin antibody. Stimulation with G17 induced an increase in the level of β-catenin protein extracted from TX-100 -insoluble fraction and reached the highest at 12 h, while a reduction in the level of β-catenin protein extracted from TX-100 solution fraction. But the responses stimulated by G17 were blocked by L365,260 (Right lane: L).