Toxicological study of the hepatotherapeutic herbal formula, Chunggan extract, in beagle dogs

Table 3 Serum chemistry analysis after administration of Chunggan extract for 4 wk

Number of dogs	Male (12)				Female (12)
CGX (mg/kg per day)	0	100	200	400	0	100	200	400
AST (IU/L)	37.0 ± 3.4	36.3 ± 2.0	40.3 ± 14.2	41.3 ± 8.3	40.6 ± 8.5	34.3 ± 5.7	37.0 ± 4.3	40.0 ± 9.5
ALT (IU/L)	57.6 ± 7.3	48.6 ± 8.1	49.3 ± 29.7	65.6 ± 24.0	38.0 ± 7.0	46.6 ± 16.2	44.0 ± 1.7	33.6 ± 9.0
ALP (IU/L)	328 ± 46	299 ± 102	390 ± 221	238 ± 74	271 ± 104	391 ± 93	377 ± 159	174 ± 43
BUN (mg/dL)	18.2 ± 1.3	20.1 ± 3.1	22.8 ± 3.6	19.3 ± 3.5	13.4 ± 1.9	17.6 ± 2.6	13.9 ± 3.9	24.4 ± 4.8a
Creatinine (mg/dL)	1.05 ± 0.1	1.20 ± 0.1	1.23 ± 0.1	1.18 ± 0.1	1.03 ± 0.2	1.15 ± 0.1	1.09 ± 0.1	1.11 ± 0.1
Glucose (mg/dL)	99.0 ± 15.5	115.6 ± 5.1	112.3 ± 11.9	110.7 ± 4.2	114 ± 14	121 ± 10.2	117 ± 5.6	116 ± 26.1
Total chol. (mg/dL)	252 ± 30	223 ± 34	260 ± 25	217 ± 51	299 ± 73	296 ± 34	263 ± 24	261 ± 58
Total bilirubin (mg/dL)	0.23 ± 0.1	0.27 ± 0.1	0.23 ± 0.1	0.30 ± 0.1	0.30 ± 0.1	0.30 ± 0.0	0.30 ± 0.1	0.33 ± 0.1
Total protein (g/dL)	7.83 ± 0.2	7.37 ± 0.2	7.33 ± 0.2	7.37 ± 0.3	7.60 ± 0.3	7.47 ± 0.6	7.40 ± 0.4	7.53 ± 0.2
Albumin (g/dL)	4.73 ± 0.3	4.33 ± 0.2	4.47 ± 0.1	4.60 ± 0.4	4.77 ± 0.3	4.50 ± 0.3	4.63 ± 0.3	4.57 ± 0.2
AG ratio	1.53 ± 0.2	1.43 ± 0.2	1.60 ± 0.2	1.67 ± 0.3	1.70 ± 0.1	1.50 ± 0.1	1.70 ± 0.1	1.57 ± 0.1
C.phosphokinase (IU/L)	133 ± 15	130 ± 39	171 ± 89	157 ± 48	199 ± 30	116 ± 24	155 ± 37	144 ± 23
Triglyceride (mg/dL)	25.0 ± 3.0	35.6 ± 24.8	30.0 ± 3.6	287 ± 7.2	36.0 ± 16.6	28.4 ± 9.5	36.0 ± 5.2	47.3 ± 17.1
Calcium (mg/dL)	14.8 ± 0.5	14.5 ± 0.5	14.6 ± 0.3	14.4 ± 0.2	14.5 ± 0.5	14.7 ± 0.9	14.1 ± 0.4	14.6 ± 0.7
Inorganic pho. (mg/dL)	3.33 ± 1.1	4.43 ± 0.3	4.23 ± 0.3	4.43 ± 0.6	3.80 ± 0.5	3.90 ± 0.6	4.03 ± 0.8	3.63 ± 0.3
LDH (IU/L)	105 ± 45.7	132 ± 73.5	91.7 ± 32.8	106 ± 60.3	80.0 ± 11.5	68.3 ± 9.7	146 ± 60.7	146 ± 126.1
GGT (IU/L)	4.63 ± 0.3	3.93 ± 1.3	4.03 ± 0.4	4.37 ± 1.3	4.93 ± 1.0	5.13 ± 0.4	4.57 ± 1.0	5.53 ± 0.5
Sodium (mmol/dL)	148 ± 2.0	146 ± 1.1	144 ± 2.1	144 ± 0.6	146 ± 1.2	148 ± 1.00	147 ± 2.0	147 ± 0.4
Potassium (mmol/dL)	4.21 ± 0.1	4.32 ± 0.1	4.31 ± 0.2	4.18 ± 0.2	4.08 ± 0.23	3.95 ± 0.2	4.24 ± 0.3	4.42 ± 0.4
Chloride (mmol/dL)	116 ± 1.1	115 ± 2.5	115 ± 2.3	116 ± 0.6	117 ± 1.5	114 ± 1.5	114 ± 3.2	114 ± 1.5

After administration of Chunggan extract (CGX) for 4 wk, serum chemistry was analysed to compare CGX-treated groups to the control group. AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; ALP: Alkaline phosphatase; BUN: Blood urea nitrogen; AG ratio: Albumin globulin ratio; LDH: Lactate dehydrogenase; GGT: Gamma glutamyl transferase.

^aP < 0.05 among BUN in female grops treated with 0 mg, 100 mg, 200 mg and 400 mg CGX.